Composite
E.ssb_Bst

Part:BBa_K3790162:Design

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-18)


E.ssb-(G2S)3-Bst


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

3-5-E.ssb-bst	5-ATTAACACCACGGCTTGCGCCGCTGCTGTGATGATG                       
5-3-E.ssb-bst	5-GCAAGCCGTGGTGTTAATAAAG                                     
E.ssb-3-5-bst	5-CCGCCGCTACCGCCAAACGGGATATCATCATCAAAATCCATAG                
E.ssb-5-3-bst	5-GTTTGGCGGTAGCGGCGGTAGCGGCGGTAGCGCTGAGGGCGAAAAGCCG


Source

References